Biodegradasi Phenanthrene oleh Bakteri Indigenous Laut Pari Kepulauan Seribu

The increasingly of oil pollution in the offshore will be threaten the aquatic life and the ecosystem. Polycylic Aromatic Hydrocarbon (PAHs) phenanthrene is one of crude oil′s component which is potentally carcinogenic and persistent in nature environment. That\'s the reason why we need a technology that offers great promise to resolve the oil pollution, called bioremediation. Role of indigenous bacteria will be important when we choose biostimulation process. Main step of this research are isolation of indigenous Pari sea\'s bacteria capable of degrading phenenthrene, ability reconfirm test and growth test, molecular identification bacteria based on 16S rRNA gene sequences, phenanthrene biodegradation using TOC analyzer. Two bacteria capable of degrading phenanthrene were identified as Ruegeria sp DG898 and Alpha proteobacterium GMDJE10F1. Alpha protobacterium GMDJE10F1 is a cosmopolit bacteria, which can optimally grows on temperature 25 oC and 2 persen salinity and µ = 0,118 h-1 . Ruegeria sp DG898 is a tropical seawater bacteria which can optimally grows on temperature 25 oC and 2 persen salinity and µ = 0,2099 h-1 . After 19 days, biodegradation of phenanthrene by Ruegeria sp DG898 was 70 persen and Alpha proteobacterium GMDJE10F1 was 65.23 persen. Overall biodegradation phenanthrene by Ruegeria DG898 have more better result than Alpha proteobacterium GMDJE10F1. Berkala Penelitian Hayati (Journal of Biological Researches). Edisi Khusus No. 4F : 13-18.