Characterization of Amylase from Marine Bacteria

In the previous study, we have isolated marine microbes which have capability to hydrolyze carbohydrate such as amylum, amylopectin, mannan, xylan and cellulose. In this study, we focused on marine bacteria which can produce extracellular amylase. Extracellular enzymes that produced by Brevibacterium sp. Have been collected with the enzyme activity 1.26 U/mL. Precipitation the enzymes using ammonium sulphate showed the enzyme recovery around 41 persen. The purified enzyme that collected from purificatin process using gel filtration chromatography Sephadex G-75, was characterized for pH and temperature optimum condition. The results showed that pH optimum on 6.4 and temperature optimum on 50oC. Proceedings International Conference on Biotechnology. Biotechnology Bridging Biodiversity to Industry Bogor ISSN / ISBN / IBSN : 978-602-98275-2-1