Kultur Tunas Pucuk Anggrek Endemik Terancam Punah Cymbidium Hartinahianum J.B. Comb & R.E Nas.

The history of Cymbidium hartinahianum, a terrestrial Indonesian endemic orchid was dated back to 1976 when Rusdi Nasution and J.B. Comber was first discovered and described it as a new species. The rare endemic orchid C. hartinahianum was successfully rediscovered by Bogor Botanic Garden expedition team in 2004, after more than a decade of searching. Human activities are a significant threat to the remnant habitat which destruction could occur at any time. To date the future of this orchid depends on successful axenic culture in the laboratory. Shoot tip culture of this orchid has been carried out in Bogor Botanic Garden using Murashige & Skoog (MS) and Knudson C (KC) basal medium supplemented with organic compound, 5 mgtl Benzyl Amino purine (BAP) and 3 mgtl ZA (Zeatin). New shoots were formed after 3 weeks, where overall optimum growth occur on KC compared to MS medium. New shoots and roots have formed in KC medium without hormone and organic compound, proven the effectiveness of this medium to induce the growth of the species. Addition of 5 mg/I BAP on to the medium has doubled the number of new leaves. The highest number of new shoot (5) and leaves (15) was given by KC supplemented with 5 mgtl BAP. Prosiding Seminar Nasional: Peran Biosistimatika. Purwokerto, 12 Desember 2009. Hal. 471-476