The DNA Sequence Performance of COI Gene in White Cockatoos (Cacatua, Psittaciformes)

Performance of nucleotide sequencing of 807-bp segments of mitochondrial c cytochrome oxidase I (COI) was analyzed to study the relationship and grouping of six species of white cockatoos: Cacatua galerita, C. sulphurea, C. alba, C. moluccensis, C. sanguinea, and C.goffini. Two species (Aprosmictus erythropterus and Prioniturus platurnus) were used as outgroups in this study. The sequences contained a mean composition of 25.9 % tymine, 30.8 % cytosine, 26.0 % adenine, and 17.4 % guanine. Based on Kimura 2-parameter analyses, the genetic distance between individuals within a species (intraspecific) ranged from 0.0000 (C. alba) to 0.0026 ± 0.0012 (C. galerita) and the genetic distance between individuals of different species ranged from 0.0299 ± 0.0057 (C. sulphurea vs C. galerita) to 0.0991 ± 0.0120 (C. moluccensis vs C. sanguinea). Sequence variations and haplotypes were found in Cacatua. In total, 196 (%) variable sites were identified with 189 sites being parsimoniously informative. Neighbor-joining (NJ) and maximum parsimony (MP) analyses showed two main groups in Cacatua: (C. sanguinea + C. goffini), and (C. alba + C. moluccensis) + (C. galerita + C. sulphurea). Treubia, Vol. 37. Hal. 1-14 ISSN 0082-6340