The Need for True Green Solvent to Assure Environmental Friendly Biopesticide in Neem Plant (Azadirachta indica A. Juss) Crude Extract Formulation

Neem plant, Azadirachta indica possess considerable potential as pesticide alternatif due to its environmental safety level. Problem arise in the formulation of neem plant crude extract using solvent which is not environmentally friendly. Purpose of the research is an examination of neem plant crude extract formulated by geen solvent which is known to be more secure to environmental and human health. Te previous experiment of acute oral toxicity of 5000 mg/kgBW neem plant crude extract formulation with green solvent as solvent had yielded visible clinical signs after 1 hour treatment namely anxiety, weakness, asphyxiate, and hyperlacrimation. These clinical signs were followed by 40 % animal test lives through. Hence, according to Annex 3 of 420 OECD the level of administered dose which culminates on ≥ 40 % mortalities is lethal. It encourages the defeasance of green solvent in the following remaked formulation. The test is conducted on 2 – 3 weeks DDY mices in three groups of threatment namely control group, surfactant threatment group, and formulated neem without solvent and the mortality rate was recorded through 14 days observation. No clinical sign is observed and 100 % of survival rate in all three groups. The 6th International Symposium for Sustainable Humanosphere. Bogor, 15-16 November 2016. Hal. 219-225