Kultur Spora In Vitro Tiga Varian Pakis Simpei Cibotium Barometz

The golden chicken fern, Cibotium barometz (L.) J. Sm., is an important Indonesian export commodity for both traditional and modern medicine. This trade commodity still relies on harvest from the wild, therefore this species has been included in Appendix II of the Convention on International Trade in Endangered Species (CITES). Morphological and population studies of C. barometz in Sumatra have been conducted in 5 provinces. The results showed that C. barometz of Sumatra consists of three variants which are mainly distinguished by hair colour, namely ‘golden yellow’, ‘golden brown’, and ‘pale or white’. The purpose of this studywas to compare the rate of growth and development of the gametophytes of three variants of C. barometz in vitro culture. Six genotypes of the three variants were germinated via in vitro spora culture. Spores were sown on a ½ Murashige & Skoog (1/2MS) media with the addition of BAP (0, 2, 4 dan 6 mg/L) and NAA (0; 0.01; 0.03 dan 0.05 mg/L). A ½ MS without BAB and NAA was used for subculture. Sporophyte induction used MS, ½ MS, and ¼ MS with the additon of NAA (0; 0.5 dan 1 mg/L), in a factorial arrangement. First germination occurred 14 days after sowing. For all variants, the highest numbers of rhizoid and filamentous stages were observed on ½ MS media with the additon of 2 mg/L BAP with or without the addition of 0.01 mg/L NAA. For two variants (golden yellow and golden brown) the highest number of sporophytes occured on ¼ MS without NAA. The highest number of sporophyte formation occured onone genotype of one variant, namely the ‘golden brown’. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia vol.1 No.7 Hal. 1649-1653 ISSN 2407-8050