Study on The Effect of Pollutants on The Production of Aaptamines and The Cytotoxicity of Crude Extract from Aaptos Suberitoides

This experiment was conducted to study the effects of anthropogenic stressor on the spatial variability of secondary metabolites from marine sponge Aaptos suberitoides. Samplings were conducted at 7 sites in Marine National Park of Thousand Islands that are extended within 30 km off Jakarta bay on late February 2011. Sponges were collected and quantified by means of liquid chromatography coupled with photo-diode array detection, whereas, cytotoxicity of sponges extracts was determined against T47D (breast) cancer cell lines. Results showed that the spatial production of aaptamine and isoaaptamine did not correlate with the quality of their surrounding habitat, despite nitrite and nitrate levels significantly affected the bioactivity of crude extracts. Squalen, Vol. 7, No. 3. Hal. 97-104