Actinoplanes Bogoriensis Sp. Nov., A Novel Actinomycete Isolated From Leaf Litter

A novel actinomycete, designated LIPI11-2-Ac043T, was isolated from leaf litter collected in Indonesia. According to phylogenetic analysis based on the 16S rRNA gene sequence comparisons, strain LIPI11-2-Ac043T was closely related to Actinoplanes abujensis A4029T (99.3 persen) and Actinoplanes brasiliensis DSM 43805T (98.8 persen). Spores of strain LIPI11-2-Ac043T were motile and the sporangia were spherical. The predominant menaquinone was MK-9(H6) and the principal polar lipids were phosphatidylinositol, phosphatidylethanolamine, phosphatidylglycerol and diphospatidylglycerol. The whole-cell sugars were galactose, glucose and mannose; rhibose, arabinose and xylose were also detected as minor components. The major fatty acids were anteiso-C15:0, iso-C16:0, iso-C15:0 and anteiso-C17:0. These data supported the affiliation of strain LIPI11-2-Ac043T to the genus Actinoplanes. Meanwhile, the results of DNA–DNA hybridization and physiological and biochemical tests indicated that strain LIPI11-2-Ac043T can be distinguished from its closest related species. Therefore, strain LIPI11-2-Ac043T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes bogoriensis sp. nov. is proposed. The type strain is LIPI11-2-Ac043T (=InaCC A522T=NBRC 110975T).2014 Impact Factor 1.730* The Journal of Antibiotics, Vol. 69. 2016. P: 26–30