Screening and study of antifungal activity of leaf litter actinomycetes isolated from Ternate Island, Indonesia

Objective: To assess the antifuchnagraal catcetriivziety a obfu ancdtainnocme yocfe lteeas fi sloitltaetre sa catgianionmsty Cceatneds idfrao mal bTiecrannast,e S Iasclcahnadr oamndyc teoscerevisiae (S. cerevisiae), and Aspergillus niger. Methods: Actinomycetes were isolated from leaf litter of Durio species, Syzygium aromaticum, Piper betle, Myristica fragrans, or Pandanus species and unknown plants. Actinomycetes isolates wuseirneg c Bueltuurrye-dK iinrb ay lmiqeutihdo dm ewditihu mm.o Bdiiofiaccattiivoen c. oMminpiomuunmds iwnheirbei teoxrt rcaocntecden atnradt itoens taendd a cgealiln lset afkuanggeasl uwseirneg cmoonldeuccutleadr .s eAqcutiennocme ydcaetate isn t1h6aSt rpRrNoAdu gceende .the highest antifungal activity were indentified Results: Out of 50 selected isolates, two isolates MG-500-1-4 and SR-2-2 has highest activity iaognasi nasnt dS m. coerrpehvoislioagei.c aClo onbcseenrtvraattiioonns oifn dmicaateterdia lt hcaotn etxatirnaicntgs nofu cMleGi-c5 a00c-id1s-,4 p arnotde iSnRs-, 2C-a2+ caanuds eKd+ cell leakage and invagination of S. cerevisiae cells. Based on 16S rRNA gene identification, MG- 5re0s0p-e1c-t4iv aenlyd. SR-2-2 isolates are similar to Streptomyces misakiensis and Streptomyces tricolor Conclusions: Ternate Island contains interesting biodiversity of actinomycetes that has potential use in agriculture, fisheries, and human health to reduce problem of fungal pathogen. Asian Pacific Journal of Tropical Medicine. 2014. volume 7(Suppl 1): S238-S243.