Badan Riset dan Inovasi Nasional
07-11-2022
13-08-2024
302c7536-4808-4fdd-9898-8e47757e4faa
Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR)...
Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae...
The use of tetraploid banana in triploid banana breeding has been conducted thro...
The genetic diversity assessmnet of Hoya purpureofusca Hook.f. (Apocynaceae: Asc...
Indonesia and South East Asia is the center of origin and genetic variability of...
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Analisa Genetik Pisang Hibrid Diploid Berdasarkan Marka RAPD
Banana breeding by crosses is one way to get new cultivars with a better quality. This study aims to verify molecular charact eristic ofdiploid hibrid bananas i.e MDRK, SNMT and MDMT using Random Amplified Polymorphic DNA (RAPD) markers. Ten RAPD primershave been selected to detect the differences in molecular pattern from all three diploid hybrid bananas. The results showed t hat the hybridcombination of MDRK, SNMT and MDMT produced bands that shared some similarities with each parents by 43.83 persen, 51.25 persen and 47.21 persenrespectively as the number of bands from type I, III and IV markers. Type III marker is the important marker for identificati on of maleparents. Type VII marker (non-parent bands) was detected from hybrid MDRK, SNMT and MDMT by 18 (20.22 persen), 6 (7.5 persen) and 11(12.64 persen) respectively. Marker of type VII can be used to identified hybrid because they are present only in offspring. The highestpolymorphic bands was obtained by SNMT (83.9 persen) and the lowest obtained was performed by MDMT (71.25 persen). From this result, RAPDmarkers can be used as a tool to detect the differences of molecular pattern of diploid hybrid bananas. Berita Biologi Vol. 16 No. 3. 2017. P :257-264.