Analisis Keragaman Genetik Kentang Hitam [Plectranthus rotundifolius (Poiret) Sprengel] Berdasarkan Marka ISSR Dan RAPD

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living insome parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity.This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter SimpleSequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initiallyscreened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835)generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20 persen (UBC807 and 834) to 16.39 persen (OPB 10) with an average of 12.50 persen polymorphism. Clustering analysis was performed based on ISSR andRAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity amongaccessions was 51-100 persen to which most of the accessions were clustered with more than 80 persen similarity. This confirmed our hypothesis ofthe low level of variation existed among accessions. Berita Biologi 2014 13(2):127-135