Biooxidation Of Berberine By The Endophytic Fungus Coelomycetes AFKR-1 Isolated From Kayu Kuning(Archangelesia Flava L. Merr.: Menispermaceae)

Totaly eighteen kinds of endophytic fungi have been isolated from the youngstems of kayu kuning [ Archangelisia flava(L.) Merr.]collected from Bogor BotanicGarden. All of isolated fungi have been screened for their capability to transform the host plant alkaloid berberine in two kinds of liquid mediums, potato dextrose broth (PDB) andglucose-yeast extract-peptone (GYP) respectively. Biotransformation reactions weremonitored and analyzed by a normal phase thin layer chromatography technique. Thescreening process revealed that the fungus Coelomycetes AFKR-1 could be able totransform berberine into a biotransformed product in PDB medium. Scaling-up biotransformation reactions (5 x 200 ml medium in 500 ml size Erlenmeyer) which wereincubated at room temperature with agitation at 120 rpm yielded 20 % conversion rate of substrate into a product in 48 hrs. The chemical structure of biotransformed product was proposed as a 7- N -oxide-berberine based on their spectroscopic data. Proceeding International Seminar Biotechnology for Enhancement the Tropical Biodiversity P1-P6, Tahun 2011. link: https://www.scribd.com/document/41384477/Day-1-Fullpaper-Poster