Chemical Profile by LC-MS/MS and Some Bioactivities from Leafs of Kolowe (Chydenanthus excelsus): A Wild and Rare Plant from Indonesia

Secondary metabolites from Kolowe leaf (C. excelsus) with chemical screening are saponins and flavonoids, spread in fraction methanol, ethyl acetate, and a water fraction. Profile secondary metabolites of methanol and ethyl acetate extract from an analysis of LC-MS/MS indicated twenty compounds with molecular masses ranging from 226-1064 for fraction of methanol, suspected of ten types of saponin and compound 10 as flavonoid glycosides. Furthermore, ethyl acetate fraction contained eight compounds with a molecular mass range at 239-1383 consisting of fifty-three compounds saponins and flavonoids. Methanol crude extract, extract a fraction of methanol and ethyl acetate potentially cytotoxic with value LC50 < 30 ppm against A. salina and has an antioxidant activity with value LC50 < 50 ppm against DPPH. In addition crude extract methanol, methanol fraction and the ethyl acetate fraction to be antimicrobial against 19 types of microbes that against gram positive and gram negative bacteria as well as against some fungi.