Penapisan Mikroba Penghasil Enzim untuk Biotransformasi Senyawa Nitril.

Screening of Pontential Microbes as Enzyme Producer for Nitrile Biotransformation. A number of potential microbes have been isolated from contaminated area by toxic compound. The isolates were assayed to know best consisted of capability in degradation of acetonitrile. Screening of microbe was done with microtitter plate method based on growth ability tested by INT (Iodonitrotetrazoliumchloride) reagent and product of the degradation was determined by GC. Five isolates of bacteria (TPIK, GLB5, LP3, 26 A2, and 23 A2) capable of utilizing acetonitrile as sole source of carbon and nitrogen. Isolate bacteria of TPIK was potential for bioremediation compared to the other isolates because the isolate the lowest residual acetonitrile as much as 89,76 mM from initial concentrationof 1000 mM. Isolate bacteria of TPIK had biotransformation pattern in parallel, between growth, activity, and ammonium as biotransformation product. Potential microbes had capability for transformation of nitrile compound were TPIK, LP3, and GLB5. The objectives of this study were screen of microbes as enzyme producer for biotransformation of nitrile,and biotransformation patterns of the microbes to acetonitrile. Berkala Penelitian HAYATI (Journal Biological Researches). Edisi Khusus No. 4F : 13-18