Badan Riset dan Inovasi Nasional
07-11-2022
12-08-2024
56f9d07a-4518-4dcd-b540-7bfe25571ffa
𝛽-Galactosidase is enzyme which hydrolyze lactose to glucose and galactose, asl...
A natural based nanocomposite film consisting of chitosan, montmorillonite (MMT)...
Polyurethane-modified epoxy (PME) has been synthesized. Synthesis was conducted ...
The objective of this study is to fabricate and characterize the anisotropic bon...
Abstract. In the present study, we have fabricated isotropic bonded Pr-Fe-B magn...
INFORMASI: Data berikut ini masih dalam proses pemenuhan Prinsip SDI.
Purification And Characterization Of Streptomyces Sp. IK Chitinase
Streptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2 persen (w/v) colloidal chitin, fermented for 96 hours at 300C. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatography. On SDS-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a mass of 71 kDa. Chitinase was optimally active at pH of 6.7 and at 370C. Km value and Vmax of the protein for colloidal chitin were 2.92 mg/ml and 4.26 μg/h, respectively. Indonesian Journal of Biotechnology Vol.15, No.1. June 2010. Page: 29 – 36.