Review of Fast Technics for Isolation and Structure Elucidation of Triterpene Saponins Compound from Bionatural Products

Saponins have specific characteristics are highly polar, generally, as a compound isomers, diversity of this compounds founded with a large number in living natural material, complex chemical structure and physicochemical properties of the compound is very similar to one another. Characteristics such as a major cause difficulty separation of the compound and the difficulty in determining the molecular structure by conventional techniques. Triterpene saponins for the separation more difficult because more polar, as well as the elucidation of the structure because the structure is more complex. isolation Technique of saponins most rapidly and precisely locate compound saponin saponin extraction using methanol-water (7: 3 or 1: 1), the purification of saponin mixture with the addition of ether solvent in a solution containing saponin fractions, isolation techniques by preparative HPLC or semi- preparative reversed-phase HPLC eluent with the determination of the analytical column; and for the determination of the structure quickly and accurately using 1H and 13C NMR-2D frequency ≥ 400 MHz techniques DEPT, HH-COSY, HMBC, HMQC / HSQC, ROESY-2D, ROE-1D, TOCSY-1D, and spectrometry FTIR and FABMS like ESIMS