Badan Riset dan Inovasi Nasional
07-11-2022
12-08-2024
a2c5f3f6-fcb7-403a-85b7-41b3ffa5d788
Two Lactobacillus plantarum strains, InaCC B122 and InaCC B128, isolated from In...
Mannanase has been high potential for industry application, such as pulp bleachi...
𝛽-Galactosidase is enzyme which hydrolyze lactose to glucose and galactose, asl...
β-Galaktosidase merupakan enzim penghidrolisis laktosa menjadi glukosa dan galak...
In this study would be investigated the effects of partial evaporation and chemi...
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Hydrolysis of UHT milk lactose by partially purified crude enzyme of β-galactosidase obtained from Lactobacillus plantarum B123 indigenous strain
Crude enzyme of β-Galactosidase obtained from Lactobacillus plantarum B123 indigenous strain after partial purification, by ammonium salt precipitation and membrane filtration, was investigated to hydrolyze lactose in UHT milk at batch process. The β-galactosidase activity contained in the enzyme at 4.8 and 7.2 U/mL milk were applied for the hydrolysis in two types of UHT milks, full cream and low fat milks, with their respective total fats 3.5 and 1.0 persen w/v. The highest degree of lactose hydrolysis in full cream milk was 60.0 persen of its initial lactose concentration, achieved by the application of 7.2 U β-galactosidase/mL milk for 9 hat 50°C. The hydrolysis in low fat milk could be at 81.1 persen under the same condition. Enzyme activity, hydrolysis time and total fat concentration in milk affected the lactose hydrolysis (P ≤ 0.05). The β-galactosidase contained in the partially purified enzyme has a potential use for commercial production of low lactose milk. International Food Research Journal 22(6): 2274-2279 (2015)